A fatigue detection apparatus is provided. The fatigue detection apparatus includes a first detector, a second detector and a processor. The first detector is configured to obtain a first physiological signal and the second detector is configured to obtain a second physiological signal. The processor is coupled to the first detector and the second detector, obtains a plurality of characteristic time differences according to the first physiological signal and the second physiological signal, and determines a fatigue detection result according to a variation trend of the obtained characteristic time differences varying with time. Furthermore, a fatigue detection method is also provided.
