The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid (LA 18:2 ω-6) to eicosadienoic acid (EDA 20:2 ω-6) and/or α-linolenic acid (ALA 18:3 ω-3) to eicosatrienoic acid (ETrA 20:3 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in oleaginous yeast are disclosed.