Provided are an enterovirus D68 (EV-D68) or a modified form thereof, or anucleic acidmolecule comprising a genomic sequence or cDNA sequence of the EV-D68 or amodified formthereof, or a complementary sequence of the genomic sequence or cDNA sequence,or apharmaceutical composition comprising the EV-D68 or a modified form thereof,or the nucleicacid molecule, and use of the EV-D68 or a modified form thereof, or thenucleic acid moleculein the manufacture of a pharmaceutical composition for treating a tumor.