HANNAH, L., Curtis,GEORGELIS, Nikolaos,SHAW, Janine, R.,BOEHLEIN, Susan, Kim
申请号：
USUS2014/047879
公开号：
WO2015/013444A1
申请日:
2014.07.23
申请国别(地区):
WO
年份:
2015
代理人:
摘要：
Materials and methods for enhancing heat stability of AGPase by mutating amino acids within the large subunit of AGPase are disclosed. Polynucleotides that encode a mutant large subunit of AGPase provide for increased heat stability when expressed with AGPase small subunit. In one embodiment, the mutant large subunit has a single amino acid mutation. Exemplified mutant large subunits include those of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO:16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, and SEQ ID NO:52. In further embodiments, the mutant large subunit comprises two or more amino acid mutations. An exemplified mutant large subunit comprising two or more amino acid mutations is designated as SH2N131R:C424V having an amino acid sequence shown in SEQ ID NO:23. In another exemplified embodiment, a mutant large subunit of the invention, designated herein as SH2-E, comprises 8 amino acid mutations.Linvention concerne des matières et des procédés permettant dobtenir une stabilité accrue à la chaleur de lAGPase, par une mutation dacides aminés dans la grande sous-unité de lAGPase. Des polynucléotides codant pour une grande sous-unité mutante de lAGPase confèrent une stabilité accrue à la chaleur quand ils sont exprimés avec une petite sous-unité de lAGPase. Dans une forme de réalisation, la grande sous-unité mutante comporte une seule mutation dacide aminé. Les grandes sous-unités mutantes données à titre dexemple comprennent celles de SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO:16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ
