PROBLEM TO BE SOLVED: To provide a method for exploring a gene with a function which increases a content of protein in a seed, and to provide a method for increasing the content of protein.SOLUTION: The invention relates to a method for increasing a content of protein in a seed by expressing, within a plant, a transcription factor consisting of a chimeric protein with transcriptional repressor activity which is obtained by fusing a transcription factor consisting of a protein comprising a specific amino acid sequence and a functional peptide converting any transcription factor into a transcriptional repressor factor, or a protein comprising a specific amino acid sequence.COPYRIGHT: (C)2015,JPO&INPIT【課題】種子におけるタンパク質含量を増加させる機能を有する遺伝子の探索及び前記タンパク質含量の向上方法の提供。【解決手段】特定のアミノ酸配列を含むタンパク質からなる転写因子と任意の転写因子を転写抑制因子に転換する機能性ペプチドとを融合させた、転写抑制因子活性をもつキメラタンパク質、若しくは特定のアミノ酸配列を含むタンパク質からなる転写因子を植物内で発現させることにより、種子のタンパク質含量を向上させる方法。【選択図】なし