An invention discloses Lyme disease vaccine by obtaining high immune response against surface protein BB069 from Borrelia burgdorferi, the causative agent of Lyme disease.Present invention includes a protein comprising amino acids identical at least 80% to modified Borrelia burgdorferi BB0689 protein (as set forth in SEQ ID 5), chemically coupled to bacteriophage Qβ virus-like particles. Said invention also relates with a method of production and purification of protein, comprising amino acids identical at least 80% to modified Borrelia burgdorferi BB0689 protein as set forth in SEQ ID 5.
