Provided are an isolated T-cell receptor, a modified cell, an encoding nucleic acid and the use thereof. The isolated T-cell receptor (TCR) comprises at least one of an [alpha] chain and a [beta] chain, both the [alpha] chain and the [beta] chain comprising a variable region and a constant region, and is characterized in that the T cell receptor can specifically identify the antigen Her2/neu expressed by tumor cells, and the amino acid sequence of the variable region of the [alpha] chain has at least 98% identity with the amino acid sequence as shown in SEQ ID NO: 1, and the amino acid sequence of the variable region of the [beta] chain has at least 98% identity with the amino acid sequence as shown in SEQ ID NO: 2. The TCR can specifically identify a tumor antigen and, at the same time, can prevent any possible off-target toxic and side effects. Immune cells modified with the TCR have significant antitumor effects.本發明提供了一種分離的T細胞受體、其修飾的細胞、編碼核酸及其應用。該分離的T細胞受體（TCR）包括α鏈和β鏈中的至少一者，該α鏈和β鏈均包含可變區和恆定區，其特徵在於，該T細胞受體能夠特異性識別腫瘤細胞所表達的抗原Her2/neu，並且該α鏈的該可變區的氨基酸序列具有與SEQ ID NO: 1所示的氨基酸序列至少98%的一致性，該β鏈的該可變區的氨基酸序列具有與SEQ ID NO: 2所示的氨基酸序列至少98%的一致性。該TCR可特異性識別腫瘤抗原，同時可避免可能的脫靶毒副反應。用此TCR修飾的免疫細胞具有顯著的抗腫瘤效果。
