Min Dong,Lisheng Peng,Pål Erik Gustav Stenmark,Ronnie Per Arne Berntsson
申请号：
US14403768
公开号：
US09598685B2
申请日:
2013.03.13
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Disclosed herein are botulinum neurotoxin (BoNT) polypeptides with a modified receptor binding domain of Clostridial botulinum serotype B (B-Hc), comprising one or more substitution mutations corresponding to substitution mutations in serotype B, strain 1, V1118M； Y1183M； E1191M； E1191I； E1191Q； E1191T； S1199Y； S1199F； S1199L； SI 20 IV； or combinations thereof. Specific combination mutations include E1 191M and S1199L, E1191M and S1199Y, E1191M and S1199F, E1191Q and S1199L, E1191Q and S 1199Y, or E 1191 Q and S 1199F. Other substitution mutations are also disclosed. Isolated modified receptor binding domains, chimeric molecules, pharmaceutical compositions, and methods of using the same are also disclosed.
