The U.S.A.; as represented by the Secretary; Department of Health and Human Services
发明人:
Mitchell Ho,Nan Li,Dimiter S. Dimitrov
申请号：
US16322712
公开号：
US20200216558A1
申请日:
2017.07.20
申请国别(地区):
US
年份:
2020
代理人:
摘要：
A panel of human variable heavy (VH) single domain monoclonal antibodies specific for cell-surface glypican-2 (GPC2) are described. Methods for the diagnosis and treatment and GPC2-positive cancer are also described. Recombinant immunotoxins comprised of a GPC2-specific VH domain antibody and a clinically used form of Pseudomonas exotoxin A (PE38) were generated and shown to inhibit GPC2-positive neuroblastoma tumor cell growth and inhibit neuroblastoma xenograft growth in nude mice, without significant toxicity. Chimeric antigen receptors comprising a GPC2-specific VH single domain antibody are also described. T cells expressing the GPC2-specific CARs potently killed GPC2-positive neuroblastoma cells in a dose-dependent manner.
