COMPOSITION FOR PREVENTION OR TREATMENT OF LIVER DISEASES CONTAINING LINE OF VEGETABLE STEM CELLS RECEIVED FROM PANAX GINSENG CAMBIUM, INCLUDING WILD GINSEN OR STEAM GINS
ДЗИН Юнг Ву (KR),ЛИ Ын Куонг (KR),ЛИМ Мин Чун (KR)
申请号:
RU2011109257/15
公开号:
RU2011109257A
申请日:
2009.08.14
申请国别(地区):
RU
年份:
2012
代理人:
摘要:
1. Composition for the prevention or treatment of liver diseases, containing a line of cells derived from cambium Panax ginseng, having the following characteristics, and / or their extract and / or lysate and / or culture:! (a) they are in a naturally undifferentiated state; ! (b) they are a homogeneous cell line; and! (c) they are morphologically characterized by multiple vacuoles. ! 2. The composition for the prevention or treatment of liver diseases according to claim 1, in which the cell line is further characterized in that:! (a) it exists at the level of single cells in suspension cultivation; ! (b) it has a low sensitivity to shear stress in the bioreactor compared to cell lines originating from other tissues than cambium Panax ginseng; and! (c) it has a high growth rate and can be cultured more stably compared to cell lines originating from other tissues than cambium Panax ginseng. ! 3. The composition for the prevention or treatment of liver diseases according to claim 1, in which the cell line is obtained by the isolation method, comprising the following stages:! (a) obtaining tissue containing cambium Panax ginseng; ! (b) cultivating the obtained cambium-containing tissue in a medium containing indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA), thereby inducing a cell line originating from cambium, while the osmotic is fed to the cambium-containing storage root tension before, during or after cultivation; and! (c) collecting the cambium-derived cell line. ! 4. Composition for the prevention or treatment of liver diseases according to any one of claims 1 to 3, in which Panax ginseng is wild1. Композиция для профилактики или лечения заболеваний печени, содержащая линию клеток, происходящих из камбия Panax ginseng, обладающих следующими характеристиками, и/или их экстракт и/или лизат и/или культуру: ! (a) они находятся в естественно недифференцированном состоянии; !(b) они представляют собой однородную линию клеток; и ! (c) они морфологически хара