Method for screening an agent being useful for the treatment of dry eye and/or corneal and conjunctival lesion and a pharmaceutical composition obtained by the method
Disclosed herein is a method for screening a drug which is efficacious in treating dry eye with a dry eye severity level 3 or higher that is defined in 2007 report of the International Dry Eye Workshop and/or keratoconjunctival disorders, and the use of compounds identified by the method. The method comprises preparing a rabbit model of corneal and conjunctival lesion having corneal and conjunctival epithelial abrasion by instilling n-heptanol solution optionally including ethanol to an eye of a rabbit and administering a test agent to the eye of the rabbit model and determining an effect of restoring corneal tissue of the test agent, wherein the conditions of cornea and conjuctiva are observed with a dye such as fluorescein. In one embodiment, the compound identified by the method is human serum albumin.
