DAOUST MARC ANDRE,LAVOIE PIERRE OLIVIER,VEZINA LOUIS PHILIPPE,COUTURE MANON
申请号：
IN152/CHENP/2014
公开号：
IN2014CN00152A
申请日:
2014.01.07
申请国别(地区):
IN
年份:
2015
代理人:
摘要：
A method of producing a virus like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid into the plant or portion of the plant. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a native rabies virus structural protein. The nucleotide sequence may further comprise one or more than one amplification element. Optionally a second nucleic acid might be introduced into the plant or portion of the plant. The second nucleic acid comprising a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a matrix protein for example but not limited to a rabies matrix protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids thereby producing the VLP.
