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Method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme
专利权人:
发明人:
Petra Siegert,Stefan Evers,Marion Merkel,Nina Mussmann,Hendrik Hellmuth,Timothy OConnell,Karl-Heinz Maurer,Ulrich Schwaneberg,Felix Jakob,Ronny Martinez,Brian Laufs,Ayhan Aydemir,Thomas Weber
申请号:
US14199569
公开号:
US09695461B2
申请日:
2014.03.06
申请国别(地区):
US
年份:
2017
代理人:
摘要:
The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.
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中国工程科技知识中心
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