OTTEN GILLIS,DONNELLY JOHN JAMES,ULMER JEFFREY,POLO JOHN M.,BARNETT SUSAN,SINGH MANMOHAN,DUBENSKY THOMAS W JR.,OHAGAN DEREK
申请号：
IN270/KOLNP/2003
公开号：
IN250654B
申请日:
2003.03.03
申请国别(地区):
IN
年份:
2012
代理人:
摘要：
A method of producing a microparticle having an adsorbent surface to which a vector construct capable of expressing a selected nucleic acid sequence is adsorbed, said method comprising the steps of: (a) emulsifying a mixture of a polymer solution and a detergent to form an emulsion, wherein the polymer solution comprises a polymer selected from the group consisting of a poly(α- hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and a polycyanoacrylate, wherein the polymer is present at a concentration of about 1% to about 30% in an organic solvent, and wherein the detergent is present in the mixture at a weight to weight detergent to polymer ratio of from about 0.00001:1 to about 0.5:1 (b) removing the organic solvent from the emulsion, to form said microparticle and (c) adsorbing the vector construct to the surface of the microparticle, wherein said vector construct is selected from the group consisting of an ELVIS vector and an RNA vector construct.
