Алексей Владимирович,Антон Геннадьевич,Владимир Георгиевич,Роман Николаевич,Веремеев, Алексей Владимирович,Кутихин, Антон Геннадьевич,Нестеренко, Владимир Георгиевич,Болгарин, Роман Николаевич
申请号：
EA201992185
公开号：
EA201992185A1
申请日:
2017.12.04
申请国别(地区):
EA
年份:
2020
代理人:
摘要：
The present invention relates to medical biotechnology, medicine, traumatology, orthopedics, dentistry and orthodontics. The proposed method for producing a biological matrix designed to replace defects in bone tissue can include several successive stages, such as pretreatment of biological material, rough cleaning and fractionation, deep cleaning and extraction, delipidation, fermentation, demineralization and sterilization in supercritical media. The obtained bioresorbable biological matrix is characterized by increased osteo- and biointegration, optimal biodegradation rate, high biocompatibility, lack of immunoreactivity from the recipient, high ability to osteoconduction, expressed osteogenic potentials during osteosynthesis and bone grafting. The matrix consists of bone collagen, hydroxyapatite and / or calcium phosphate and is distinguished by the fact that bone collagen is presented in a native unreduced form with a fully preserved three-dimensional structure, hydroxyapatite and calcium phosphate are presented in a native amorphous form, and the matrix itself, purified from cell debris, is foreign lipids, nucleic acids and immunogens, contains residual amounts of bone morphogenetic proteins and is impregnated with vesicular phosphatidylcholine and / or cholesterol with included gelatin (hydrolyzed collagen), and / or bone atelocollagen, and / or poly - (- caprolactone) and additionally included biologically active substances (including bioactive peptides and growth factors).Настоящее изобретение относится к медицинской биотехнологии, медицине, травматологии, ортопедии, стоматологии и ортодонтии. Предложенный способ получения биологического матрикса, предназначенного для замещения дефектов костной ткани, может включать несколько последовательных стадий, таких как предварительная обработка биологического материала, грубая очистка и фракционирование, глубокая очистка и экстракция, делипидирование, ферментация, деминерализация и стерилизация в сверхкритических ср
