The present disclosure provides a humanized monoclonal antibody, including: a light chain variable region； and a heavy chain variable region, wherein a nucleotide sequence encoding the amino acid sequence for the light chain variable region including a nucleotide sequence which encoding the amino acid sequence of SEQ ID NO. 9 or an amino acid sequence with at least 80% sequence identity to the sequence of SEQ ID NO. 9, and a nucleotide sequence encoding the amino acid sequence for the heavy chain variable region including a nucleotide sequence which encoding the amino acid sequence of SEQ ID NO. 10 or an amino acid sequence with at least 80% sequence identity to the sequence of SEQ ID NO. 10, and wherein the humanized monoclonal antibody binds to CD34.本發明提供一種人源化之單株抗體，包括：一輕鏈變異區；以及一重鏈變異區，其中編碼該輕鏈變異區之胺基酸序列的核苷酸序列包括一核苷酸序列，其編碼序列辨識號：9之胺基酸序列或者是與序列辨識號：9之序列具有至少80%之序列相似度的一胺基酸序列，而編碼該重鏈變異區的之胺基酸序列的核苷酸序列包括另一核苷酸序列，其編碼序列辨識號：10之胺基酸序列或者是與序列辨識號：10之序列具有至少80%之序列相似度的一胺基酸序列，又其中該人源化單株抗體與CD34結合。
