Ana Andréa Teixeira de Carvalho,Aryadina Mara Ribeiro de Souza,Hilário Cuquetto Mantovani
申请号：
BRPI1001695
公开号：
BRPI1001695B1
申请日:
2010.05.24
申请国别(地区):
BR
年份:
2019
代理人:
摘要：
process to increase hc5 bovine production and its use in the conservation of fruit juices. The object of the patent application is a process that allows up to 80% increase in activity of bovine hc5 after consecutive cultivation of streptococcus bovis hc5 in culture medium with specific composition. The maintenance of culture ph under acidic conditions during the growth of the microorganism resulted in an increase of bovine hc5 production by more than 90% compared to the control treatment. The use of sugarcane juice as an alternative substrate for the production of bovine hc5 increased the volumetric productivity of the peptide 4 times. After growth in culture medium plus sugarcane juice, the cells had a volumetric productivity of 2560 u / ml / h while in the control treatment using glucose as carbon source (16 g / l) the volumetric productivity was 640 ua / ml. ml / h. The use of bovine hc5 inhibits growth and reduces the cell viability of alicyclobacillus acidoterrestris dsmz 2498 in different juices. The thermal resistance of spores was also lower with the addition of bovine hc5. acidic extracts of bovine hc5 added to juices at a dose of 66.6 water / ml and 80 water / ml inhibit growth and increase the thermal sensitivity of spores of this microorganism, respectively.processo para aumento da produção de bovicina hc5 e o uso da mesma na conservação de sucos de frutas. o objeto do pedido de patente trata-se de um processo que permite obter aumento de até 80% na atividade da bovicina hc5, após o cultivo consecutivo de streptococcus bovis hc5 em meio de cultura com composição específica. a manutenção do ph da cultura em condições acídicas durante o crescimento do microrganismo resultou no aumento da produção da bovicina hc5 em mais de 90%, comparado ao tratamento controle. o uso de caldo de cana como substrato alternativo para a produção da bovicina hc5 aumentou a produtividade volumétrica do peptídeo em 4 vezes. após o crescimento em meio de cultivo acrescido de cald
