The present invention is directed to a kit for evaluating on the skin of a patient the sensitivity to penicillin, comprising (A) a first vial containing a major determinant mixture, the major determinant mixture comprising benzylpenicilloyl polylysine； (B) a second vial containing lyophilized minor determinant mixture, the minor determinant mixture comprising a lyophilized mixture of neutralized (1) penicillin G potassium； (2) penicilloic acid； and (3) penilloic acid； (C) a third vial containing amoxicillin sodium； and (D) instructions for carrying out a method to evaluate on the skin of a patient the sensitivity to penicillin. The present invention is also directed to a method to rule out penicillin allergy using the disclosed kit.
