PROBLEM TO BE SOLVED: To provide method of preparing a model animal of ovarian clear cell carcinoma which can evaluate a therapeutic agent or a preventive agent of ovarian clear cell carcinoma.SOLUTION: A method of preparing a model animal of ovarian clear cell carcinoma according to the invention includes: a transplant process for transplanting cells of ovarian clear cell carcinoma derived from humans in an abdominal cavity of an immunodeficient animal other than human a recovery process for recovering cancer cells from ascites of the immunodeficient animal accumulating the ascites after the transplant process a cloning process for cloning the cancer cells obtained in the recovery process to establish a cancer cell strain a selection process for selecting the cancer cell strain that has a cell proliferation rate that is higher by at least 1.4 times and has a VEGF production amount that is higher by at least 3 times compared to the cells of the ovarian clear cell carcinoma derived from humans that are used for the transplant out of the cell strain obtained in the cloning process and a peritoneal dissemination process for performing peritoneal dissemination by transplanting the cancer cell strain selected in the selection process in the abdominal cavity of the immunodeficient animal other than human.SELECTED DRAWING: NoneCOPYRIGHT: (C)2019,JPO&INPIT【課題】本発明は、卵巣明細胞がんの治療剤又は予防剤を評価可能な卵巣明細胞がんモデル動物を作製する方法を提供することを目的とする。【解決手段】本発明は、ヒト以外の免疫不全動物の腹腔内にヒト由来の卵巣明細胞がん細胞を移植する移植工程と、上記移植工程後に腹水を貯留した上記免疫不全動物の腹水からがん細胞を回収する回収工程と、上記回収工程で得られたがん細胞をクローン化し、がん細胞株を樹立するクローン化工程と、上記クローン化工程で得られた上記細胞株の中から、移植に使用した上記ヒト由来の卵巣明細胞がん細胞と比較して細胞増殖率が1.4倍以上高く、かつ、VEGF産生量が3倍以上高いがん細胞株を選抜する選抜工程と、上記選抜工程で選抜されたされたがん細胞株をヒト以外の免疫不全動物の腹腔内に移植して腹膜播種する膜播種工程と、を備える、卵巣明細胞がんモデル動物の作製方法を提供する。【選択図】なし
