PROBLEM TO BE SOLVED: To provide an embryo inspection method of a zebra fish capable of realizing a practically usable inspection accuracy and inspection time.SOLUTION: An excitation light source 3 is provided obliquely above a fish egg tray 2 arranged with a number of wells 20 accommodating one embryo 10 of a zebra fish together with the water, and a fluorescence detector 4 is provided immediately above the fish embryo tray 2. A highly accurate determination between a normal egg and an abnormal egg can be made based on an optical spectrum intensity of a peak wavelength or a narrow channel alone in the vicinity thereof from among the fluorescence spectra acquired from the embryo of the zebra fish after five to eight hours elapsed from the conception using an inspection device for inputting a signal voltage outputted from the fluorescence detector 4 into a determination part 5 for the embryo. The high-speed and high accuracy quality determination can be realized using a multi-well plate.SELECTED DRAWING: Figure 1【課題】実用可能な検査精度および検査時間を実現可能なゼブラフィッシュの受精卵検査方法を提供する。【解決手段】ゼブラフィッシュの１つの受精卵１０を水とともに収容した多数のウエル２０を配置した魚卵トレイ２の斜め上方に励起光光源３を設け、魚卵トレイ２の直上に蛍光検出器４を設ける。蛍光検出器４から出た信号電圧を受精卵判定用の判定部５に入力する検査装置を用いて、受精から５-８時間経過したゼブラフィッシュの受精卵から得られた蛍光スペクトルのうちピーク波長又はその近傍の狭帯域だけの分光スペクトル強度に基づいて正常卵と異常卵との高精度の判別ができる。マルチウエルプレートを用いることにより、高速かつ高精度の良否判定が実現できる。【選択図】図１
