The present invention relates to a method of evaluating multiple plant indiuifluals or plant lines for deep rooting property under the same culture medium moisture conditions- A method of the present invention is a method of evaluating multiple plant Individuals for deep rooting property under the same culture medium moisture conditions, by planting multiple plant individuals together in a sufficiently deep culture vessel and lowering the culture medium moisture content gradually- The present invention also provides a method for evaluating plants for deep rooting property, wherein a culture vessel is installed in a water tank so that the culture medium moisture content can be readily adjusted, and thus management of cultivation conditions is facile and efficient. It is possible to select a plant, which can grow in dry and low-groundwater level environment efficiently, by using the method of the present invention. Thus, the method of the present invention can find application in breeding a crop suitable for dry land cultivation and trees usable in desert greening-