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TROUSSE POUR LA PRODUCTION D'UN ÉCHANTILLON BIOLOGIQUE RENDU TRANSPARENT, ET PROCÉDÉ DE PRODUCTION D'UN ÉCHANTILLON BIOLOGIQUE RENDU TRANSPARENT
专利权人:
KANAZAWA MEDICAL UNIVERSITY
发明人:
HATTA, TOSHIHISA,UCHISHIBA, MAIMI,HIGASHI, NOBUAKI,SHIMADA, HIROKI,SHIMAMURA, ERIKO
申请号:
EP13850135
公开号:
EP2926658A4
申请日:
2013.10.30
申请国别(地区):
EP
年份:
2016
代理人:
摘要:
Provided are: a method and a kit for producing a biological specimen clear, which enable the clearing within a short time and by which a wide variety of biological specimens can be cleared; and a method and a kit for preserving a cleared biological specimen. A method for producing a cleared biological specimen, comprising steps as mentioned in items (A) to (C). (A) Step (1) of treating a material to be cleared with a fixing solution that is an aqueous solution containing paraformaldehyde or the like, a nonionic surfactant (at a concentration of 1% or more) and an alkali and optionally containing a buffering agent to produce the material that is cleared is involved. (B) Step (2) of treating the material that is produced in step (1) or a material to be cleared which is produced by a conventional fixing method with a clearing accelerator solution that is an aqueous solution containing a nonionic surfactant (at a concentration of 1% or more) and an alkali to produce the material that is cleared is involved. (C) Step (3) of treating the material produced in step (1) or (2) with a preservation solution that is an aqueous solution containing a nonionic surfactant (at a concentration of 1% or more) and a polyhydric alcohol to produce the material that is cleared is involved. A kit which can be used in the method.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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