A separation system comprising a blood separation device containing a controller , a red-blood-cell (RBC) detection module, and a pump driver； and a cartridge configured to be reversibly attached to the blood separation device, the cartridge including: (i) a first container configured to contain a blood sample, wherein the first container includes a first opening； (ii) a tubing in fluid flow communication with the first opening； (iii) a pump in fluid flow communication with the tubing, wherein the pump is configured to move a fluid from the first container to the pump and to contain a fluid； and (iv) a check valve mounted to the tubing, wherein the check valve is configured to allow a fluid to flow from the first container to the pump and to not allow a fluid to flow from the pump to the first container.一種分離系統包含有：一含有一控制器、一紅血球(RBC)偵測模組以及一泵驅動器的血液分離裝置；以及一被配置成要被可逆地附接至該血液分離裝置的匣，該匣包含：(i)一被配置成用以含有一血液樣品的第一容器，其中該第一容器包含一第一開口；(ii)一與該第一開口呈流體流動連通的配管；(iii)一與該配管呈流體流動連通的泵，其中該泵被配置成用以將一流體從該第一容器移動至該泵以及用以含有一流體；以及(iv)一被安裝至該配管的止回閥，其中該止回閥被配置成用以容許一流體從該第一容器流動至該泵而且不容許一流體從該泵流動至該第一容器。
