Klaus Dornmair,Reinhard Hohlfeld,Jorg Prinz,Katherina Siewert,Song-Min Kim
申请号：
US13814263
公开号：
US09678061B2
申请日:
2011.08.05
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The present invention relates to a method of identifying a target antigen of T cells comprising (a) contacting (aa) cells expressing (i) a functional T cell receptor complex comprising predefined matching T cell receptor α and β chains； and (ii) a read-out system for T cell activation； with (ab) antigen-presenting cells carrying (iii) peptide libraries encoded by randomised nucleic acid sequences； and (iv) MHC molecules recognised by the T cell receptor of (i)； (b) assessing T cell activation using said read-out system； (c) isolating antigen-presenting cells that are in contact with the cells in which the read-out system indicates T cell activation； (d) identifying the target antigen or the nucleic acid molecule encoding said target antigen.
