Use of 3’-hydroxygenistein is disclosed. The 3’-hydroxygenistein has an anti-oxidation capability. Therefore the 3’-hydroxygenistein can be used to manufacture compositions for anti-oxidation.本發明提供一種3’-羥基染料木黃酮之用途,所述3’-羥基染料木黃酮具有抗氧化之功效。藉此,所述3’-羥基染料木黃酮可用於製備抗氧化的組合物。