Clinical application of Dsg3 in head and neck cancer: prediction of cancer malignancy, method as molecular application target and RNA interference sequence application method for specific inhibition of Dsg3 expression
CHANG-GUNG MEMORIAL HOSPITAL;CHANG GUNG UNIVERSITY
发明人:
张东杰,張東杰,郑恩加,鄭恩加,陈盈汝,陳盈汝
申请号：
TW095140849
公开号：
TWI377952B
申请日:
2006.11.03
申请国别(地区):
TW
年份:
2012
代理人:
摘要：
The present invention relates to a clinical application of DSG3 molecule in head and neck cancers for predicting degree of cancer malignancy, using as method of molecular application targets, and RNA interference sequence application method of specific inhibition of DSG3 expression, especially to prove that the highly expressed DSG3 gene relates to the size, depth, and metastasis of tumor by comparative analysis of highly expressed DSG3 in tumor tissue and clinical feature of cancer cells. Thus, the highly expressed gene may be used as clinical application, estimation of the degree of tumor malignancy, or application of molecular targets for head and neck cancers. Furthermore, the present invention is aiming at the DSG3 molecule in the cells to design a RNA interference sequence specific to mRNA of DSG3 to effectively inhibit the expression of DSG3 protein, the growth, invasion, and metastasis of the cancer cells.一種DSG3分子於頭頸癌之臨床應用－預測癌症惡性程度及作為分子應用標的之方法與專一性抑制DSG3表現之RNA干擾序列運用方法，針對DSG3在腫瘤組織中之表現過高與癌細胞的臨床表徵做對照分析，證實該基因之過高表現與腫瘤之大小、深度及轉移有相關，因此該基因過高表現可用於臨床應用、估算腫瘤惡性程度、或針對頭頸癌之分子標的物之應用。此外本發明針對細胞內DSG3分子，設計一段專一性對DSG3 mRNA的RNA干擾序列，有效地抑制DSG3蛋白之表現，進而抑制癌細胞的生長、侵犯及轉移。
