Nanotechnology is an emerging field that manipulates materials in the atomic and molecular levels. Nanotechnology finds potential applications in almost all fields wherein biomedical application plays a vital role in our day to day life. The herbal leaves and its extracts have many biomedical applications which are evident from our ancient time. The processing of herbal nanoparticles from medicinal plants have increasing widespread medical applications. The plants like Azadirachta indica, Acalypha indica and Tridax procumbens have various medicinal properties. The nanoparticles have been obtained from Azadirachta indica leaves for biomedical applications due to their high anti microbial activity. The particle size obtained from Azadirachta indica leaves have been in the range of 1 - 100 nm. The morphology of the Azadirachta indica nanoparticles have studied through TEM and AFM studies. The Azadirachta indica nanoparticles have been coated on the textile fabrics employing the standard technique. The different characterisation studies have been carried out to confirm the presence of nanoparticles on textile fabrics. The antibacterial study has been made on the Azadirachta indica nanoparticles coated and uncoated textile fabrics. The coated fabrics shows an improved anti bacterial activity than uncoated fabrics against E.coli and S. aureus. Thus, the Azadirachta indica nanoparticles coated fabrics can be used in hospitals during surgery to avoid contamination and also for wound dressing. The proposed method is ecofriendly, cost effective and easily avoids infections. Keywords: Nano herbal particles, fabrics, Anti bacterial activity.