植物色素体への遺伝子導入法

• 收藏
• 分享

专利权人:

独立行政法人農業生物資源研究所

发明人:

田部井 豊,蒲池 伸一郎,市川 裕章,中村 英光

申请号：

JP2008075181

公开号：

JP5320782B2

申请日:

2008.03.24

申请国别(地区):

JP

年份:

2013

代理人:

摘要：

PROBLEM TO BE SOLVED: To provide a method for transferring a gene into a plant pigment genome, and to provide a transformed plant body with the objective gene transferred into a pigment genome by the above method.

SOLUTION: A green callus is induced from a rice seed transferred with a gene so as to express transcriptional factor OsGLK1 gene functioning to induce differentiation into chloroplast. Subsequently, a foreign gene is transferred into a plant cell by particle gun method to effect gene transfer into the pigment genome. Next, a callus having the foreign gene is selected and redifferentiated to obtain a transformed plant body where the foreign gene is transferred into the chloroplast genome. That is, by using, in advance, a tissue where the gene of a transcriptional factor inducing the differentiation to chloroplast is overexpressed, in a plant material subject to chloroplast transformation, the tissue where the chloroplast is differentiated and presents green color can be transferred with a gene, succeeding improving gene transfer efficiency.

COPYRIGHT: (C)2010,JPO&INPIT

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/