The present invention aims to provide a novel composition for improving intestinal flora and a method for improving intestinal flora that can increase the ratio of good bacteria in the intestinal flora. The present invention relates to a composition for improving intestinal flora containing isoxanthohumol and/or xanthohumol as an effective ingredient.本發明係以提供一種可增加腸內菌叢中的好菌之存在比率的新穎的改善腸內菌叢之組成物及改善腸內菌叢之方法為目的。本發明係有關於一種包含異黃腐醇及/或黃腐醇作為有效成分的改善腸內菌叢之組成物等。