A one step all-in-one apparatus for body fluid sampling and sensing, comprising: a housing(1), a plurality of integrated sampling and sensing assemblies, a cartridge(12), sealing films(101,102), an upper cover(13), a sampling port(6), a linear actuating mechanism and a control circuit, with every integrated sampling and sensing assembly comprising a micro fluid-sampling needle(2), a needle hub(3) and a test strip(4)； the micro fluid-sampling needles(2) penetrate the skin to sample the body fluid and deliver the body fluid to the test strips(4) directly, and changes occurred on the test strips(4) are read by the sensor(5), wherein sampling and sensing are completed in one step. The sealing films(101,102) and a plurality of chambers(15) of the cartridge(12) compose sealed reaction chambers(15) and the linear actuating mechanism which is connected with the control circuit electrically drives the integrated sampling and sensing assemblies allowing the micro sampling needles(2) to penetrate the skin and reach the designated depths under the skin. The one step all-in-one apparatus for body fluid sampling and sensing has advantages as small size, high efficiency, convenient operation, short time consumption, less fluid consumption, depth-controllable penetration, and accurate and reliable results.
