D'AOUST, MARC-ANDRE,LAVOIE, PIERRE-OLIVIER,FALCON DE LONGEVIALLE, ANDEOL,DAOUST, MARC-ANDRE
申请号：
CA3037523
公开号：
CA3037523A1
申请日:
2017.09.29
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a GDP-4-dehydro-6-deoxy-D-mannose reductase (RMD) the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.
