Provided is a method for preparing a specimen for extensively analyzing a resident skin bacterium and/or a substance present in the stratum corneum, such as a protein or a lipid, or observing the shape of the stratum corneum.A method for preparing a specimen for analyzing a resident skin bacterium and/or a substance present in the stratum corneum or observing the shape of the stratum, comprising applying a solution containing a polyvinyl alcohol polymer to the skin so as to form a thin-film and peeling and collecting the formed thin-film.