Present invention provides specially design and fabrication of 3D Bioplotted Biphasic composite scaffold made from Collagen Treated Hydroxyapatite-Tricalciumphosphate scaffolds for the heeling of Bone tissue defects. Scaffold printed in a layer by layer fashion with 0o 45o 90o 135o lay down pattern is discussed in the present work. After high-temperature sintering scaffold was characterized by (FTIR) Fourier Transform Infrared Spectroscopy, (SEM) Scanning Electron Microscopy, (XRD) X-Ray diffraction, Porosity analysis and Mechanical testing. Scaffold samples were tested for its ability to induce cytotoxicity in Balb/c 3T3 cells at in vitro condition using elution method. Skin sensitization potential of scaffolds was evaluated in male guinea pigs using guinea pig maximization test (GPMT). Further, scaffolds were implanted in eight rabbit tibia bones and biocompatibility and histological evaluations were carried out after 4 and 8 week implantation periods. In-vitro results include bonding, surface morphology, phases, porosity, mechanical strength and Cytotoxicity. In-vivo results include sensitization, capsule formation, inflammation, presence of polymorphonuclear cells, giant cells, plasma cells, X-Rays and degradation of the material. Following invention is described in detail with the help of Figure 1 of sheet 1 showing 3D-Bioplotting of HA/TCP ceramic and Figure 2 of sheet 2 showing Sintered HA/TCP scaffolds.
