<;P>;PROBLEM TO BE SOLVED: To efficiently produce physiologically active substance-containing microparticles. <;P>;SOLUTION: A method for producing the physiologically active substance-containing microparticles is provided, which comprises a step of preparing an emulsion by mixing a physiologically active substance-containing volatile organic acid aqueous solution or a volatile organic aid solution of a physiologically active substance with a biodegradable polymer-containing volatile organic solvent, and a step of mixing the resultant emulsion with an aqueous solution of a polymer bearing negative electric charge. <;P>;COPYRIGHT: (C)2010,JPO&INPIT