Macfarlane Burnet Institute for Medical Research and Public Health Limited
发明人:
申请号：
EP17854239.5
公开号：
EP3519424A1
申请日:
2017.09.22
申请国别(地区):
EP
年份:
2019
代理人:
摘要：
A method of preparing extracellularly assembled higher order antigen from a native lower order antigen the method comprising the following steps: (i) contacting lower order antigen with a solution comprising a reducing agent for a time and under 5 conditions sufficient to reduce one or more native cysteines； and (ii) removing or diluting the reducing agent or contacting the reduced lower order antigen with an oxidising agent, to elicit assembly of lower order antigen from (i) into an assembled higher order antigen； wherein at least 10% of the lower order antigen is converted to higher order antigen in step (ii) and whereby the assembled higher order antigen 10 displays at least reduced binding to non-neutralizing antibodies compared to the lower order antigen and retains binding to at least one neutralizing antibody. A method of producing a vaccine composition comprising following the steps of the method and then mixing the assembled higher order antigen with a pharmaceutically or physiologically acceptable diluent, carrier or adjuvant. A composition comprising a 15 higher order extracellularly assembled antigen, wherein the assembled antigen displays at least reduced binding to a non-neutralizing antibody compared to a native control higher order antigen. Use of the assembled higher order antigen to stimulate an immune response or for the detection and/or isolation of an immune cell such as a B-cell specific for the antigen.L'invention concerne un procédé de préparation d'un antigène d'ordre supérieur assemblé de manière extracellulaire à partir d'un antigène d'ordre inférieur natif, le procédé comprenant les étapes suivantes : (i) mise en contact d'un antigène d'ordre inférieur avec une solution comprenant un agent réducteur pendant une durée et sous cinq conditions suffisantes pour réduire une ou plusieurs cystéines natives ； et (ii) élimination ou dilution de l'agent réducteur ou la mise en contact de l'antigène d'ordre inférieur réduit avec un agent oxydant, po
