The present invention discloses a humanized anti-EGFR antibody L4-H3 and coding gene thereof. The antibody is composed of a heavy chain and a light chain, wherein the amino acid sequence of the heavy chain variable region is shown as positions 1-145 of SEQ ID NO: 3, the heavy chain constant region is the heavy chain constant region of the human antibody IgG1, and the amino acid sequence of the light chain is shown as SEQ ID NO: 1.