A fragile substance storage transportation and release device (220) comprises a frame for inserting cartridges (224) to hold the fragile substance; a propulsion unit (222) for propelling the fragile substance out of successive cartridges, cartridge by cartridge; and an opening mechanism (228) for opening each cartridge one by one in coordination with a propulsion mechanism. The device is useful for distribution of fragile insects such as mosquitoes and there is a mechanism for automatic collection of insects from pupae.