최상원;CATHOLIC UNIVERSITY OF DAEGU INDUSTRY ACADEMIC COOPERATION FOUNDATION;대구가톨릭대학교산학협력단;CHOI, SANG WON
发明人:
CHOI, SANG WON,최상원,KIM, EUN OK,김은옥,LEE, YU JIN,이유진,SON, WOO RIM,손우림
申请号：
KR1020120014926
公开号：
KR1020130093370A
申请日:
2012.02.14
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: A production method of anti-liver toxicity, ant-diabetic, anti-aging, and anti-oxidative dihydroflavonol is provided to mass produce the dihydroflavonol by fermenting mulberry wine. CONSTITUTION: Mulberries are fermented and aged to obtain mulberry wine. The mulberry wine is mixed with ionized water with the same amount. The mixed solution is mixed with ethyl acetate for fractioning and separating supernatant. The ethyl acetate fraction is dissolved in a 20% ethane solution, and settled for one day to obtain supernatant. The supernatant is passed through a Diaion HP-20 ion resin for absorbing a flavonol compound, eluted by using a 50% ethanol solution, and concentrated to obtain a mulberry wine extract. ODS-A and Sephadex LH-20 column chromatography are successively performed to the mulberry wine extract.본 발명은 오디와인으로부터 항고혈압, 항간독성, 항당뇨 및 항노화 효능이 우수한 디하이드로플라보놀의 제조방법에 관한 것으로, 생오디에 이온수를 등량 가한 후 뭉개고 난 후 당과 산 그리고 효모을 가하여 1차 및 2차 발효 후 숙성하여 제조한 오디와인을 에틸아세테이트로 분획한 후 다이아이온 HP-20 흡착이온수지, ODS-A 및 Sephadex LH-20 칼럼 크로마토그래피를 순차적으로 실시하여 항고혈압, 항간독성, 항당뇨 및 항노화 기능성 디하이드로플라보놀 성분인 디하이드로미리세틴(dehydromyricetin, DHM), 디하이드로퀘세틴(dehydroquercetin, DHQ) 및 디하이드로캄페롤(dehydrokaempferol, DHK)를 제조하는 방법을 개시한다.
