Keith Leonard March,Brian H. Johnstone,Yansheng Du
申请号:
US15860187
公开号:
US20180187159A1
申请日:
2018.01.02
申请国别(地区):
US
年份:
2018
代理人:
摘要:
Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury.