There is disclosed a device (1) for use in artificial insemination of a domestic animal, in particular a sow (2). The device has a holder (3) for supporting a sperm container (4). The device includes a longitudinal support means (5) extending across the back of the animal to a point (7) behind the animal. Furthermore, it includes a U-shaped bracket means (8) with two resilient bracket branches (9) which are connected with a connecting means (17). During insemination, the resilient bracket branches (9) will bear on the back and sides of the sow.In order to adapt the squeezing force to sows of various sizes, the resilient bracket branches are interconnected movably via the connecting means (17). The activation means (21,22) of the connecting means enables by operation displacement of the bracket branches (9) in direction against and away from the sows sides such that a squeezing force corresponding to the squeezing force from the front legs of a boar is exerted.