A sterilization device includes: a treatment chamber with a discharge valve provided on a downstream side of the treatment chamber; a light source that irradiates a fluid stored temporarily in the treatment chamber with ultraviolet light; and a heat exchange chamber connected to an upstream side of the treatment chamber via a gate valve. The heat exchange chamber is thermally connected to the light source. The sterilization device may further include a heat pipe that thermally connects the light source and the heat exchange chamber. The heat pipe may be provided to extend vertically upward from the light source toward the heat exchange chamber.