A tubing holding device for a roller pump is disclosed. The tubing holding device is removable from the roller pump, slidably moveable and able to be opened to allow for easy exchange of tubing in roller pumps. The tubing holding device comprises a clamping mechanism that securely holds tubing in place. Tubing of various sizes may be used within a roller pump by selecting the appropriately sized tubing holding device, or by using a tubing holding device, such as one embodiment disclosed, which may be adjustable for different sizes of tubing. Roller pumps including the tubing holding devices, and methods of loading such roller pumps are also disclosed.
