Compositions and methods are provided for preventing, ameliorating, ortreating a disease caused by a species ofbacterial genus Vibrio, for example, cholera caused by V. cholerae, thecompositions containing two or more strains of lyticbacteriophage that infect and kill Vibrio cells. The bacteriophage arevirulent, which replicate intracellularly and lyse and kill thebacteria. Use of two or more strains in a single treatment, as a result of arate of mutation of the bacteria to simultaneous resistance to allof the bacteriophage to be so low as to be negligible, reduces appearance ofphage-resistant bacteria to statistical negligibility.Normal human microbial flora species were not affected. In alternativeembodiments of the method and the composition, antibioticagents or other treatment agents can be administered with a cocktail of theplurality of bacteriophage strains.
