The present invention is a Novel Multi Epitope Antigen for diagnosis of Leptospirosis sp. Leptospirosis is caused by a group of highly invasive spiral bacteria that can infect both humans and other mammals. The higher number of pathogenic serovars and serogroups are the primary reason for misdiagnosis. In this present invention the synthetically tailored multiepitope gene was designed by fusing the conserved antigenic regions of crucial outer membrane proteins, namely Lipl32, Lipl41 and Lipl21 and ompLl, two epitopes from each outer membrane proteins were linked together with tetraglycyl linkers forming 160 aminoacid sequences. This Novel multi epitope gene was designed by selecting conserved regions from more than 35 serovars, thereby making the multiepitope protein a suitable antigen candidate for detecting broad range of leptospira serovars. ELISA (Enzyme linked immune sorbant assay) and Rapid Flow through based Dot Immunoassay was standardized using Multi epitope protein as the antigen respectively
