A method for assessing pain caused by administration of a drug solution includes: anesthetizing an experimental animal； inserting a measurement electrode into skeletal muscle of the anesthetized experimental animal； puncturing an injection needle into a predetermined part of the anesthetized experimental animal while measuring a myoelectric potential of the skeletal muscle with the measurement electrode； administering a drug solution to the anesthetized experimental animal； and (i) measuring a duration time of the myoelectric response caused by the administration of the drug solution, and/or (ii) measuring an EMG intensity obtained by integrating absolute values of myoelectric potentials during a period from occurrence of the myoelectric response by the administration of the drug solution to disappearance of the myoelectric response.
