An automatic vehicle disinfection system for promoting biosecurity through application of a disinfectant to a vehicle transiting to and from an agricultural site. The automatic vehicle disinfection system including a station having one or more of opposed vehicle wash ramps, a disinfectant supply system including a pressure pump in fluid communication with a first and second riser tube, and at least one cross tube, and a control assembly including a pressure pump activation trigger configured to activate the pressure pump. The system further including a trailer hitch and a pivotable wheeled dolly configured to grip a portion of the one or more vehicle wash ramp and pivot about a wheeled axis, so as to raise at least a portion of the vehicle disinfection station off the ground for ease of transportation.
