A photo chemically activated micro bubble based root canal disinfection method for disinfecting a shaped root canal comprises (a) introducing a photoactive solution into the root canal the photoactive solution containing a photoactive compound (such as methylene blue) dissolved in an alcohol carrier solution (such as polyethylene glycol and/or ethanol) (b) removing excess photoactive solution from the root canal (c) introducing a micro bubble solution comprised of an oxygen carrier (such as perfluorocarbon) an oxidizing agent (such as hydrogen peroxide) and a surfactant (such as a nonionic polyoxyethylene surfactant) (d) sonically or ultrasonically activating the micro bubble solution in the canal and (e) introducing light into the canal.
