Disclosed are an antimicrobial traditional Chinese medicine composition, and a preparation method and a use thereof, wherein the antimicrobial traditional Chinese medicine composition is made from raw materials comprising fructus perillae, patchouli and usnea. Through the reasonable matching application and synergistic effects of traditional Chinese medicines, the extracted composition has a broad-spectrum antimicrobial effect, and is suitable for being used as a natural preservative. Experiments show that for each microorganism of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger independently, the antimicrobial effect of the matched composition of the present invention is superior to the antimicrobial effect of single plants, the result where the total effect of the composition is better than sum of the effects of single plants is achieved, and the synergistic effect of antimicrobial effects among the plants is achieved. In addition, the composition provided by the invention has the characteristics of having little flavor, a light color, a mild effect, good solubleness, being economical, having a low consumption, a high stability, and being suitable for industrial production, and the like.L'invention concerne une composition antimicrobienne à base de médicaments traditionnels chinois, et son procédé de préparation et son utilisation, ladite composition antimicrobienne à base de médicaments traditionnels chinois étant fabriquée à partir de matières premières comprenant du fructus perillae, du patchouli et de l'usnée. Grâce à une utilisation combinée judicieuse et aux effets synergiques de médicaments traditionnels chinois, cette composition à base d'extraits présente un effet antimicrobien à large spectre, et est appropriée à une utilisation en tant que conservateur naturel. Des expériences montrent que, pour chaque microorganisme parmi Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida alb
