The present invention provides a method for producing functional bio-cellulose membrane, comprising immersing a bio-cellulose membrane into a functional solution and raising a temperature to 35℃ and up a period of time. The present invention also provides a functional bio-cellulose mask produced by the method. The mask full with functional ingredient uniformly and has excellent cosmetic effect.本發明提供一種功能性生物纖維膜之製備方法,包含將一生物纖維膜浸於一功能性溶液中,並將溫度升高為35℃以上一段時間。本發明亦提供利用功能性生物纖維膜製成之功能性生物纖維面膜,其中均勻充滿功能性成分,能夠達到最佳保養效果。