A method for physiological signal analysis and its system and a computer program product storing a physiological signal analysis program are provided. Physiological signals of a subject are collected for a user to provide a detection opinion for the physiological signals in order to generate syndrome recognition parameters and syndrome weight parameters such that the collected physiological signals are analyzed and determined. The invention performs detection determination by means of combining the physiological signals of the subject and referencing to an analysis opinion from the user. Therefore, an output detection result may be believed by both doctors and patients with effectively improved accuracy of analysis result to improve the efficiency of the user in diagnosis and treatment.